IUBio

Primer design software

Agnieszka Lichanska A.Lichanska at qub.ac.uk
Wed Jan 19 12:08:23 EST 2000


Hi Scott,

I do not know if there is the best program, but I used to use Oligo 6.5
in Brisbane and I am using it now as well. It is very good and as for
commercial program not very expensive. It has a lot of nice functions if
you want to have a look at it try their Demo from http://www.oligo.net/.
I have also used MacVector but it is an expensive package and unless you
are going to use its other features it is not the best choice. The
primers designed by both are equally good.

I also tried all sorts of free software on the web.
Primer 3 from Whitehead Institute
(http://www-genome.wi.mit.edu/cgi-bin/primer/primer3.cgi) is not bad,
sometimes it might feel a bit unflexible but the new version is better.
ANGIS should have a primer design software as well which would be free.
They used to have the GCG package which comes with a primer design
software called Primer. It is good if you have X-windows program to view
the output.

I suppose that the final decision on which software to use is depending
on your needs.

Happy priming,

Agnes

-----

Dr. Agnieszka Lichanska (PhD)
Department of Medical Genetics,
Queen's University of Belfast,
BT9 7AB, Belfast, UK
Fax: +44 1232 236911
Ph: +44 1232 329241 ext. 2427
e-mail: A.Lichanska at qub.ac.uk


"Scott J. Coutts" wrote:

> Can anybody suggest some good primer design programs?
> Is there a 'clearly the best' type program in this area?
>
> --
> --------------------------------+-------------------------------------
>  Scott Coutts                   | Biological Sciences [Genetics]
>  scott.coutts at sci.monash.edu.au | Monash University
>  Phone: +61 3 9905 3872         | Wellington Rd. Clayton, Vic, 3168
> --------------------------------+-------------------------------------






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