Hello to everyone,
some weaks ago I have isolated a new plasmid from a bacterial strain and now I´m trying to carry out a characterisation of this plasmid. There´s quiete a number of enzymes which cut and I also did a number of double digestions. The problem is now to combine the fragments to a corresponding map. Unfortunately the plasmid is to large (20 kB) to sequence. Does anyone have an idea about PC-software which helps in the case that there is no further information about the sequence of the DNA? I found some files concerning a programme called "double digester" but the attempt to install it on my PC failed.
I would be very pleased if anyone could give me a hint
Institut f. Biochemie
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