You can do this as easily as a couple of mouse clicks with a freeware
program called BioEdit. It's a little big, but it installs itself and
costs $0.
BioEdit can be downloaded anonymously, installs itself, and allows you
to automatically create local NCBI nucleic acid and protein databases
and run local (or internet) Blast searches on sequences from a doc
window simply by highlighting the sequence title and clicking a menu
option. All the standard Blast programs are supported (blastp, blastn,
tblastn, blastx). It's also a fully-functional alignment editor that
reads and writes multiple formats, reads ABI trace files, does nice
shaded outputs, does several sequence manipulations, automatically runs
accessory apps, auto-links to the web, and has several other useful
features.
If you want to try it out, it's at:
http://www.mbio.ncsu.edu/RNaseP/info/programs/BIOEDIT/bioedit.html
If you don't like it, it uninstalls itself like any other Windows95/98
program.
Hope this helps,
Tom Hall
David Parkin wrote:
> Dear all,
>> I'm working with Windows versions of Lasergene and
> Vector NTI packages. I would like to know if it is
> possible to "learn" them (or olny one of them) to
> search not an Internet BLAST database at NCBI, but
> local database established with NCBI soft (blastz.exe)
> and my own sequences how it makes DNA Tools package.
> If possible, please write me how.
>> With best wishes,
>> David
>> =====
>> __________________________________________________
> Do You Yahoo!?
> Bid and sell for free at http://auctions.yahoo.com
