Dear Yin-suo
don't know about software for that, but in general look for sequences like:
Good RBS's
AGGA
AAGG
AGAGG
AGGG
Weak RBS's
AGGT
AGGC
About 5-25 bp upstream of the ATG / GTG / TTG startcodon. Sorry, you'll
still have to do some visual sequence inspection.
hope this helps
Arnoud
Yinsuo Zhao wrote in message
<3.0.32.19990131215814.006bd644 at public3.bta.net.cn>...
>Hi, Dear friends:
> I've cloned and sequenced a fragment from a kind of Gram-negative
>bacteria. I've analysed its ORF but I can't analysed the ribosome binding
>site in its promoter. Does anyone know a free or shareware in PC (or any
>internet site) could do this work?
> Thanks for your good suggestion in advance.
>>Best wishes,
>>Yin-suo Zhao
>
