Hello !
I have aligned a set of about 50 related DNA-sequences and want to
create a set of consensus primers (one direction) that should be able
to amplify (nearly) every single sequence in this alignment. There
should not be to much primers (<10) but of course the degeneracy of
each of them should also be not too high.
Does anyone know of a computer program that is able to perform this?
Thanks in advance for any help (please mail a copy of your answer to:
tbu at gmx.net) !
Thomas Burmeister