DAR USER wrote:
> We are looking at using DNA for identification of cats and dogs. Having
> talked with several manufactures, I get so many different opionions on
> what equipment is needed. We do not need/want to do any type of
> identification of potiental problems or traits or no type of research,
> only to id this dog as 'number 1' and another dog as 'number 2'.
>> 1) what is the *bare* min. equipment needed?
> 2) What is a good source of basic equipment (it can be old as long as it
> works), ie not the latest and greatest?
> 3) With DNA Identification, do we need to sequence and store all pairs or
> is there a specific subset that is unique enough for this identification?
> 4) I have found several 'free' software solutions for DNA analysis, etc.
> Is there software that will take the 'sequence' and easily store it for
> later seaches/matching?
> 5) Does anyone know of a similar project in the works?
>> Thanks in advanced,
>> Kevin
>> 6) If later, we want to take it one step farther, to determine 'dog 1' is
> the parent of 'dog 2'. How much *more* equipment and steps would be
> involved?
We are the Veterinary Service of Clinical Genetics from the Veterinary
school from Madrid (Spain). We are working in a wide range of genetic
studies, from genetic identification and paternity tests to genetic
evaluations, in pets (dogs and cats), livestock (horses, cattle and sheep)
and wild species from a few years ago. Also we sex those species of birds
which cannot be differentiated by morphology. We offer service based both on
the analysis of DNA (molecular techniques) and on the genealogical
information of the animals (quantitative techniques).
We use a sort of molecular markers called microsatellites, which have a high
polymorphism. They allow us to identify the animals and do paternity tests
with a high accuracy.
There are some different options of equipment that is needed, based on the
money you can spend, but what we advice you most is to send the samples to a
specialized lab, because the cost of this equipment is rather high. However,
the cost of an animal identification in the lab is rather low.
Anyway you can contact us for more information in the e-mail below
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Servicio de Genética Clínica
Laboratorio de Genética Molecular
Facultad de Veterinaria, UCM
E-28040 Madrid
phone: +34 1 394 3765
fax: +34 1 394 3772
e-mail: genetica at eucmax.sim.ucm.eshttp://www.ucm.es/info/genetvet
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