>>>>> "Francois" == Francois Jeanmougin <pingouin at crystal.u-strasbg.fr> writes:
Francois> In article <sc4d82yhdl3.fsf at fes6.sanger.ac.uk>, Keith
Francois> James <kdj at fes6.sanger.ac.uk> writes:
>> If you want low cost/free for small numbers of sequences try
>> Chromas to view ABI traces
>> (http://www.technelysium.com.au/chromas.html) and then ClustalX
>> for multiple alignments. You may find that ClustalX reads ABI
>> directly, but I've a feeling it doesn't.
Francois> It doesn't. Also it is designed to align
Francois> phylogenetically realted sequence and not to build a
Francois> sequencing project. Clustal is really not the solution
Francois> to build sequence contigs.
I think I phrased my reply badly. I didn't intend to suggest that
Clustal be used for assembly! I assumed that the original poster had a
reason aligning DNA (and not translating it first) e.g. sequencing a
region of the same gene from many mutants with the same primer and
wanting a representation of the different base changes observed. There
are so many features in ClustalX these days that I would not have been
surprised if it could do something unusual like that.
--
Keith James -- kdj at sanger.ac.uk -- http://www.sanger.ac.uk/Users/kdj
The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambs CB10 1SA
