Program DynaFit [P. Kuzmic (1996) Anal. Biochem. 237, 260-273]
for Windows-95/NT was updated to version 3.16.
Among other improvements in the analysis of enzyme kinetic data,
you can now plot reaction velocities in the Lineweaver-Burk
coordinates (1/[substrate] vs. 1/velocity).
An example script file using this feature is located in
./examples/Segel/Ch-IX/N/Rapid Equilibrium Random Bi Bi.txt
Here is a listing of this script, corresponding to the reaction
scheme from I. Segel (1975) "Enzyme Kinetics", page 643:
----------- 8< ---------- 8< --------------
[task]
task = simulate
data = velocities
[mechanism]
E + A <==> EA : Kia dissoc.
E + B <==> EB : Kib dissoc.
EA + B <==> EAB : Kmb dissoc.
EB + A <==> EAB : Kma dissoc.
EAB ---> E + P + Q : kp
E + P <==> EP : Kip dissoc.
E + Q <==> EQ : Kiq dissoc.
EP + Q <==> EPQ : Kmq dissoc.
EQ + P <==> EPQ : Kmp dissoc.
EPQ ---> E + A + B : k-p
[constants]
Kia = 1, Kib = 1, Kma = 1, Kmb = 1
kp = 1
Kip = 1, Kiq = 1, Kmp = 1, Kmq = 1
k-p = 1
[concentrations]
E = 0.001
A = 10
[responses]
P = 1
[progress]
rapid equilibrium
[velocity]
directory ./examples/segel/ch-ix/n/data
extension txt
mesh from 2 to 100 step 2 logarithmic
error constant 0.00002
variable B
plot Lineweaver-Burk
file p0 | concentration P = 0
file p1 | concentration P = 10
file p2 | concentration P = 20
file p3 | concentration P = 40
file p4 | concentration P = 80
file p5 | concentration P = 160
[output]
directory ./examples/segel/ch-ix/n/output
[end]
----------- 8< ---------- 8< --------------
Dowload the program from URL below...
_____________________________________________________________
Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA
pkuzmic at biokin.com * http://www.biokin.com * 608.256.1269 fax
