Does anybody know a nifty PERL or CSH script which designs primers just
like those online primer design ressources?
I would need it to design a large amount of primers which I afterwards
want to check locally by searching with FASTA trough a database to get
rid of those primers which are to similar to other sequences in the S.
The local primer design script would have the big advantage that I could
automatise the whole procedure locally on my linux box and afterwards
only have to pick the primers I like from a rather big report file.
If you do know something like this (my search in the web didn't yield
any results) please answer.
Kirsten Peter Rabitsch