IUBio

Staden Package sequencing course

James Bonfield jkb at mrc-lmb.cam.ac.uk
Mon Aug 2 08:56:30 EST 1999


In October 21st to 23rd inclusively, there is a course entitled
"Sequence Project Management Using the Staden Package".

Full details may be found on:

    http://www.ib3.gmu.edu/educate/oct.stadencourse/stadencourse.html

A summary follows:

  The course is designed to provide an introduction to the use of the
  latest version of programs produced by Rodger Staden's group for the
  management of large-scale DNA sequencing projects. The course is very
  practical. Participants will be guided through exercises during which
  they will process a substantial set of sequence data obtained from an
  ABI automatated DNA sequencers.

  In the course of the three days we will cover setting up of a new
  sequencing project, pre-processing data generated by automatic
  sequencers, creating sequencing project databases, automatic assembly
  of batches of reads into contigs, checking and editing contigs, and
  use of phred-style confidence values. Checking will include
  examination of the traces for conflicting readings, searches for
  repeated sequences and analysis of the relative positions of readings
  derived from the same template.
--
James Bonfield (jkb at mrc-lmb.cam.ac.uk)   Tel: 01223 402499   Fax: 01223 213556
Medical Research Council - Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, England.
Also see Staden Package WWW site at http://www.mrc-lmb.cam.ac.uk/pubseq/




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