Hi,
I have developped a free little software (for Windows 95 with a very simple
user interface) that is able to calculate various DNA sequence (degenerated
or not) parameters:
Melting temperature (nearest neighbors algorihtm)
Molar extinction coefficient
Molecular weight
Reverted and complemented sequence
IUPAC sequence
...
You can download it at http://area.ba.cnr.it/~e105of01/annhyb221.zip
Comments are welcome at friard at ba.cnr.it
Olivier Friard