IUBio

Multiple PCR primer analysis?

Stuart M. Brown browns02 at mcrcr.med.nyu.edu
Wed Jan 7 10:58:43 EST 1998


In article <68tg2r$isb$1 at kadri.ut.ee>, tmargus at tamm.eenet.ee (Tonu Margus)
wrote:

> Hi, 
> 
> 
> Do somebody knows, is there some program what can analyze (predict) how you
> PCR praimers are behaiving in mixture, if you are using more than two oligos
> at time.  For example you have 10 different primers in mix. 
>  
> To avoid that one primer of one pair anneals with another praimer from
> another pair.
> 
> Tõnu Margus

I have used a program called Right Primer that has multiple primer (multiplex)
analysis.  I can't say how theoretically valid this analysis might be. 



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