GeneDoc has facilities for analyzing subsets of the sequences in a
protein multiple sequence alignment for their potential as PCR sites.
It identifies positions in the alignment where all of the amino acids
within the subset being analyzed are identical and do not appear in
any of the sequences outside of the subset. There are user selectable
options that let you define a small group of amino acids to treat as
in they were a single amino acid in the analysis.
http://www.cris.com/~ketchup/genedoc.shtmlldewit at ben.vub.ac.be (mbraiban) wrote:
>Does anybody know a software that could assist me in designing primers
>for PCR recombination?
>Dept. of Virology
>Institut Pasteur de Bruxelles
>642 rue Engeland