I've heard of an easy to use, small, freeware program, which is able to
determine if a given PCR- or sequencing-primer is any good.
It should check inhibitory factors like dimers, stem-loop structures
etc. which may impair proper hybridization.
Please respond to me directly, and thanks in advance.
Peter W. Lindum, Dept. of Microbiology, DTU, Denmark.
pwl at im.dtu.dk