Maybe someone out there can help me, because I'm running out of ideas.
This is my problem : I'm trying to develop a HPLC with ELSD procedure to
quantify mannitol in allergic (? allergènes in French) drugs. My biggest
problem for the moment is a very very instable base-line (one can hardly
speek of a base-line in this case). Several parameters can be changed in
order to avoid this, but all these parameters are in correlation with
eachother. Someone in the lab suggested me to try the method of Simplex
and the aid of optimization software in order to get quickly good
results. What do you think ? Do you know where I can get such software ?
You would really help me a lot, because my boss needs the results as soon
Thank you very much for your attention and kind help.
Els Van Geldre
E-mail : Els.VanGeldre at rug.ac.be