Res. Enzyme Tth111II

[Przemko Tylzanowski]

User Name wrote:
> 
> I am looking for a restriction enzyme Tth111II. I would appreciate it if
> anyone has it or knows where I can order from to let me know.
> Margaret Ryan
> e-mail address: spbcmbr at iop.bpmf.ac.uk
Search of REBASE at New England biolabs at
http://www.neb.com/rebase/rebase.wais.html revealed the following:

ENZYME:         Tth111II          (Type II restriction enzyme)
PROTOTYPE:      Tth111II
RECOGNITION SEQUENCE:   CAARCA(11/9)
RECOGNITION SEQ CLASS:  nnnRnn
MICROORGANISM:  Thermus thermophilus strain 111
SOURCE:         T. Oshima
MICROORG TEMPERATURE: 75
# of sites on  ad2:53  lambda:49  pbr:5  phiX:11  sv40:11
ACCESSION #:       261
Tth111II is not commercially available.
    TRY CONTACTING:     
        T. Oshima
NEB Tth111II ISOSCHIZOMERS:  None.
Tth111II ISOSCHIZOMERS FROM OTHERS:  None.
RELATED REFERENCES:
- Roberts, R.J.
  Nucleic Acids Res. 9:r75-r96(1981).
- Shinomiya, T., Kobayashi, M., Sato, S.
  Nucleic Acids Res. 8:3275-3285(1980).
- Shinomiya, T., Kobayashi, M., Sato, S.
  (1980) Japanese Patent Office, Patent number JP 87062152.

Przemko