In article <kjt-2003960748260001 at pm074-20.dialip.mich.net>, kjt at umich.edu (Ken J. Takekoshi) writes:
>>> Has anyone had any experience with the Macintosh version of ClustalW?
> I'm trying to give it a go, but I'm having problems. I don't understand
> how to load in files that are in FAST format in the MAC architecture. I
> also don't really understand what the dialog box is for that comes up when
> the programs begins. Something about choosing input and output
> sources/files.
>> Thanks again.
>>> Sincerely,
>>> Ken Takekoshi
Hi Ken:
the "Mac version" of ClustalW is not really a mac version; it is just the same
old program recompiled for macs. Hence it is a bit cryptic; sorry!!
The initial dialogue is not used for anything much; just ignore it.
To get your sequences in, get them together in 1 file in TEXT or ASCII
format (if you are uusing a word processor for example). here is an
example in fasta format .... just use a ">" to delimit each sequence:
>HBB_HUMAN
VHLTPEEKSA VTALWGKVNV DEVGGEALGR LLVVYPWTQR FFESFGDLST
PDAVMGNPKV KAHGKKVLGA FSDGLAHLDN LKGTFATLSE LHCDKLHVDP
ENFRLLGNVL VCVLAHHFGK EFTPPVQAAY QKVVAGVANA LAHKYH
>HBB_HORSE
VQLSGEEKAA VLALWDKVNE EEVGGEALGR LLVVYPWTQR FFDSFGDLSN
PGAVMGNPKV KAHGKKVLHS FGEGVHHLDN LKGTFAALSE LHCDKLHVDP
ENFRLLGNVL VVVLARHFGK DFTPELQASY QKVVAGVANA LAHKYH
>HBA_HUMAN
VLSPADKTNV KAAWGKVGAH AGEYGAEALE RMFLSFPTTK TYFPHFDLSH
GSAQVKGHGK KVADALTNAV AHVDDMPNAL SALSDLHAHK LRVDPVNFKL
LSHCLLVTLA AHLPAEFTPA VHASLDKFLA SVSTVLTSKY R
>HBA_HORSE
VLSAADKTNV KAAWSKVGGH AGEYGAEALE RMFLGFPTTK TYFPHFDLSH
GSAQVKAHGK KVGDALTLAV GHLDDLPGAL SNLSDLHAHK LRVDPVNFKL
LSHCLLSTLA VHLPNDFTPA VHASLDKFLS SVSTVLTSKY R
>MYG_PHYCA
VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK
TEAEMKASED LKKHGVTVLT ALGAILKKKG HHEAELKPLA QSHATKHKIP
IKYLEFISEA IIHVLHSRHP GDFGADAQGA MNKALELFRK DIAAKYKELG
YQG
>GLB5_PETMA
PIVDTGSVAP LSAAEKTKIR SAWAPVYSTY ETSGVDILVK FFTSTPAAQE
FFPKFKGLTT ADQLKKSADV RWHAERIINA VNDAVASMDD TEKMSMKLRD
LSGKHAKSFQ VDPQYFKVLA AVIADTVAAG DAGFEKLMSM ICILLRSAY
>LGB2_LUPLU
GALTESQAAL VKSSWEEFNA NIPKHTHRFF ILVLEIAPAA KDLFSFLKGT
SEVPQNNPEL QAHAGKVFKL VYEAAIQLQV TGVVVTDATL KNLGSVHVSK
GVADAHFPVV KEAILKTIKE VVGAKWSEEL NSAWTIAYDE LAIVIKKEMN
DAA
Best of luck,
Des Higgins
