Isoelectric Point calculations

Eugene A Kapp 100277.2367 at CompuServe.COM
Fri Mar 8 01:31:35 EST 1996

I have found that the predicted pI calculations from two WWW 
sites differ by approximately 0.4 for a given protein sequence. 
Expasy (http://expasy.hcuge.ch/ch2d/pi_tool.html) and the genome 
sequencing site at (http://genome1.bio.bnl.gov/bbq.html) are 
therefore using slightly different pKR values.

Could someone suggest the correct pKR values or add some light to 
the situation.

Many thanks.

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