I have found that the predicted pI calculations from two WWW
sites differ by approximately 0.4 for a given protein sequence.
Expasy (http://expasy.hcuge.ch/ch2d/pi_tool.html) and the genome
sequencing site at (http://genome1.bio.bnl.gov/bbq.html) are
therefore using slightly different pKR values.
Could someone suggest the correct pKR values or add some light to
the situation.
Many thanks.
