Introducing silent restriction sites

Christoph Gartmann GARTMANN at IMMUNBIO.MPG.DE
Tue Apr 2 04:39:23 EST 1996

In <315F9C11.507F at mrc-lmb.cam.ac.uk> Tony writes:

> we are looking for some (preferably Mac) software to identify the 
> positions where  'silent' restriction sites can be introduced by 
> mutagenesis into DNA sequence.

If you have access to GCG you may use MAP/SILENT (VMS) or map -silent (UNIX).
In conjunction with additional switches like /MAXCUTS=1 and the program MAPSORT
it shouldn't be to difficult to find a suitable restriticon site that can
easily be detected on a gel.

   Christoph Gartmann

| Max-Planck-Institut fuer      Phone   : +49-761-5108-465   Fax: -221       |
| Immunbiologie                                                              |
| Postfach 1169                 Internet: gartmann at immunbio.mpg.de           |
| D-79011  Freiburg, FRG                                                     |
+----------- Do you know MENUE, the user environment for OpenVMS? -----------+

More information about the Bio-soft mailing list

Send comments to us at biosci-help [At] net.bio.net