Re- the binding discussions.......
I don't know if this will help, but I do binding every now and then and
analyse the data by both binding curve and scatchard with Sigma Plot. The
background binding is fit with the standard polynomial fit, and the binding
with a double rectangular hyperbola (the equation stated in another reply
earlier). To get the smooth curve through your data, at least for the
binding I do a transform using around 100 points based on the best fit
equation, these are plotted as a line only, together with your real data
represented at markers only. The same can be done with the scatchard using
data generated from the fitted binding curve.
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Darren Browning __________
Rm.116 Department of Immunology [ ______ ]
The Scripps Research Institute, [ [ ___ ]
10666 North Torrey Pines Rd. [ [ [ ]
La Jolla California [ [ [ ]
92032 [ [ ]
[__________]
Tel. (619) 554-1142
Fax. (610) 554-8483
email: dbrownin at scripps.edu
