2-Site Scatchard Redux

Darren D. Browning dbrownin at scripps.edu
Fri Sep 15 02:22:04 EST 1995

Re- the binding discussions.......

   I don't know if this will help, but I do binding every now and then and 
analyse the data by both binding curve and scatchard with Sigma Plot.  The 
background binding is fit with the standard polynomial fit, and the binding 
with a double rectangular hyperbola (the equation stated in another reply 
earlier).  To get the smooth curve through your data, at least for the 
binding I do a transform using around 100 points based on the best fit 
equation, these are plotted as a line only, together with your real data 
represented at markers only.  The same can be done with the scatchard using 
data generated from the fitted binding curve.

Darren Browning                    __________
Rm.116 Department of Immunology   [  ______  ]
The Scripps Research Institute,   [ [  ___   ]
10666 North Torrey Pines Rd.      [ [ [      ]
La Jolla California               [ [ [      ]
92032                             [ [        ]
Tel. (619) 554-1142               
Fax. (610) 554-8483
email: dbrownin at scripps.edu

More information about the Bio-soft mailing list

Send comments to us at biosci-help [At] net.bio.net