Where to find tips and advice for design of primers?

Bo Servenius bo.servenius at wblab.lu.se
Tue Nov 28 17:08:11 EST 1995

Dear Netters!

I am trying to collect advices for manual design of primers. Is there any
good netsites out there with this info or could you help me post some

I have an old one regarding purin content - it should not be more than
50% - but is this really true nowadays with pure synthesis chemicals.

Another one is you should have Gs and Cs in the 3'end but not more than 3
Gs in a row.

Than thera are the obvious ones regarding secondary structure and

Maybe you have some more advice to donate??



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