In article <4984q0$68n at shellx.best.com>,
hquinn at shellx.best.com (INTERNET ROCKHOUSE) wrote:
>: >Dear colleagues,
>: >is there any programm available for the generation of restriction sites in
>: >a given nucleotide sequence? I have to introduce several restriction sites
>: >in a nucleotide sequence by changing the codon usage without altering
>: >the amino acid sequence. Introducing these sites by hand is a very tediuos
>: >work. So, I would like to know if someone knows a computer program doing
>: >this work.
>>: If you have access to GCG, the mapping programs have a "/silent" option
>: (-silent on Unix) which will do exactly that, find silent restriction
sites.
>>Didn't realize that GCG has this function; I was looking for the same thing.
>The program 'mutsite' is part of the pc-gene software suite and does
>precisely this.
There is even better on PC: the program silmut from ftp.bio.indiana.edu. It
does a really good job, is fast and more reliable than map/silent which
sometimes is a bit confused...
--------------------------------------------------------
Dr. Gilles TRUAN
Department of Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd
Dallas, TEXAS 75206-9038
Phone # : (214) 648-4741
Fax # : (214) 648-8856
e-mail : truan at utsw.swmed.edu
--------------------------------------------------------
