: >Dear colleagues,
: >is there any programm available for the generation of restriction sites in
: >a given nucleotide sequence? I have to introduce several restriction sites
: >in a nucleotide sequence by changing the codon usage without altering
: >the amino acid sequence. Introducing these sites by hand is a very tediuos
: >work. So, I would like to know if someone knows a computer program doing
: >this work.
: If you have access to GCG, the mapping programs have a "/silent" option
: (-silent on Unix) which will do exactly that, find silent restriction sites.
Didn't realize that GCG has this function; I was looking for the same thing.
The program 'mutsite' is part of the pc-gene software suite and does
precisely this.
