In article <48sku3$815 at mserv1.dl.ac.uk>,
jpleiss at TEBIO1.BIOLOGIE.UNI-STUTTGART.DE (Juergen Pleiss) wrote:
>Dear colleagues,
>is there any programm available for the generation of restriction sites in
>a given nucleotide sequence? I have to introduce several restriction sites
>in a nucleotide sequence by changing the codon usage without altering
>the amino acid sequence. Introducing these sites by hand is a very tediuos
>work. So, I would like to know if someone knows a computer program doing
>this work.
>Thank you,
>Juergen
Yes Juergen, there is a little shareware for DOS called SILMUT, that
you can find at ftp.bio.indiana.edu. It will take your aminoacid sequence and
generate a map of possible restriction sites. You can edit the input file
containing the restriction sites and enzymes names. If you need more
information, just ask...
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Dr. Gilles TRUAN
Department of Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd
Dallas, TEXAS 75206-9038
Phone # : (214) 648-4741
Fax # : (214) 648-8856
e-mail : truan at utsw.swmed.edu
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