Hello,
I am studying a two substrate enzyme reaction and am having a little
difficulty in interpreting my data.
I have been varying both substrate concentrations and have produced
a series of plots (Double Reciprocal and a/v aginst a). From these primary
plots I have replotted the slopes and intercepts aginst the second substrate
concentration.
These later plots should (according to the literature) be straight lines
however they are not.
Does anyone know of a package, shareware, excel macros, that can be
used to analyse these more complex kinetics.
Please respond by email,
Thanks in advance,
Ian
