IUBio

Two Substrate Enzyme Kinetics

I.N.Taylor int at ukc.ac.uk
Mon May 1 09:38:01 EST 1995


Hello,
	I am studying a two substrate enzyme reaction and am having a little
difficulty in interpreting my data.
	I have been varying both substrate concentrations and have produced
a series of plots (Double Reciprocal and a/v aginst a).  From these primary 
plots I have replotted the slopes and intercepts aginst the second substrate
concentration.
	These later plots should (according to the literature) be straight lines
however they are not.

	Does anyone know of a package, shareware, excel macros, that can be
used to analyse these more complex kinetics.

Please respond by email,
	Thanks in advance,
			Ian





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