IUBio

Program to calculate isoelectric points?

Shiping Zhang ping at bnlux1.bnl.gov
Wed Jul 26 14:03:43 EST 1995


scb1 at ellis.uchicago.edu (Samuel C. Blackman) wrote:
>Does anyone know of a program (Mac, DOS, Unix) that will calculate the
>isoelectric point of a given peptide?  Thanks!

The WWW site

http://genome1.bio.bnl.gov/bbq.html

has a program doing pI calculation.  It's an implementation of the
comprehensive procedure described by Sillero and Ribeiro, Analytical
Biochem. 179, 319-325 (1989).  The following is an example output.

-ping

CEA1_ECOLI      AAs =  522,  MW =  57288.02,  pI =  9.61
 
       pH         net charge
       1           94.95
       2           94.53
       3           90.75
       4           69.56
       5           34.25
       6           20.67
       7           15.47
       8           13.34
       9            9.15
      10          -11.74
      11          -50.22
      12          -72.90

       27  D, pK =  4.0
       46  E, pK =  4.5
        1  C, pK =  9.0
       13  Y, pK = 10.0
        7  H, pK =  6.4
       60  K, pK = 10.4
       27  R, pK = 12.0
  N-terminal, pK =  8.2
  C-terminal, pK =  3.2





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