scb1 at ellis.uchicago.edu (Samuel C. Blackman) wrote:
>Does anyone know of a program (Mac, DOS, Unix) that will calculate the
>isoelectric point of a given peptide? Thanks!
The WWW site
http://genome1.bio.bnl.gov/bbq.html
has a program doing pI calculation. It's an implementation of the
comprehensive procedure described by Sillero and Ribeiro, Analytical
Biochem. 179, 319-325 (1989). The following is an example output.
-ping
CEA1_ECOLI AAs = 522, MW = 57288.02, pI = 9.61
pH net charge
1 94.95
2 94.53
3 90.75
4 69.56
5 34.25
6 20.67
7 15.47
8 13.34
9 9.15
10 -11.74
11 -50.22
12 -72.90
27 D, pK = 4.0
46 E, pK = 4.5
1 C, pK = 9.0
13 Y, pK = 10.0
7 H, pK = 6.4
60 K, pK = 10.4
27 R, pK = 12.0
N-terminal, pK = 8.2
C-terminal, pK = 3.2
