I am looking for a (free) program running on a Mac that allows me to
calculate primer hybridization conditions at various temperatures and at
different salt concentrations.
Everybody knows about the formula Tm = 4x(G+C) + 2x(A+T), but it is not
very helpful in using at conditions far from the standard ones.
If the program could estimate the difference in Tm of primers with one or
several mismatches, it would be even better.
Thank you all Peter
Peter KULMBURG (126.96.36.199 MacName: ktl110.ukl.uni-freiburg.de)
home: Tel./Fax:+49 761 808439 work: Tel.:+49 761 270/7195 Fax:/7177