hi there again,
my recent question about use of "PC-Rare" netted me several messages, all
asking for more info about this approach... some info I know follows
1/ "PC-Rare" is copyrighted by R. Griffais at the Institut Pasteur (1993).
2/ the "octamer frequency disparity" method is described in a paper by
R. Griffais et al. "K-tuple frequency in the human genome and polymerase
chain reaction" Nucleic Acids Research 19(14), 3887-3891, 1991.
3/ from this paper: "This method differs from those previously used to select
primers in that it defines a primer according to the CONTEXT [my emphasis]
in which the primer will be used, rather than from its intrinsic
thermodynamyc properties"
4/ the program prints out immediately a set of nested primers (also with the
eight 3' bases to be retained...)
5/ the only distributor seems to be:
EUROGENTECH SA (Belgium)
Parc Scientifique de la Cense Rouge
Rue Bois Saint Jean, 14
B-4102 Seraing
Belgium
Fax # --/32/41-65.51.03
(I got a Demo for Mac from them; they have Demos for PC as well; please
contact them for more info if necessary and for addresses of the EGT
distributors in other European countries (France, Germany and Netherlands)...)
