hi there,
anybody used "PC-Rare" for PCR optimization? It's quite recent (copyright
1993), but sounds excellent at least for diagnostic work ... uses octamer
frequency disparity to select primers that are specific for target (only!)...
'host' genomes available are yeast, fly, rodent, man, E. coli (their sequence
data is sufficiently well known for constructing the comparison table for
octamers...)
Greetings, Roland