Dear Friends
I have been trying to reamplify RAPD bands cut out of the agarose gel, with
the same primers as they were amplified the first time in RAPD reaction.
In many cases I get more than one amplified product (bands) when I use a
single band as template. Eventually, I will be labelling these bands with
digoxigenin once I am sure that I am getting only one product. Has anyone
seen such an amplification (two bands from the amplification of a single
band) and what he/she did to overcome this problem. Your suggestions will
be highly appreciated. Thanks in advance
Muhammad A. Lodhi
Cornell University
