Many thanks to Michael for posting the solution to my query wrt which algorithm
the PRIMER program uses to calculate Tm's (Breslauer et al [1986] PNAS 83,
3746-3750) and also elaborating on other techniques.
Thanks again Michael
Alan "It makes supporting BIONET worthwhile" Bleasby
SEQNET/EMBnet/BIONET manager
DRAL Daresbury Laboratory
Warrington WA4 4AD
UK
