In article <1994Apr18.194116.20970 at emba.uvm.edu> brianf at med.uvm.edu (Brian Foley) writes:
>From: brianf at med.uvm.edu (Brian Foley)
>Subject: Re: Plasmid construction simulation.
>Date: 18 Apr 94 19:41:16 GMT
>hosun at ALLIANT.SNU.AC.KR wrote:
>: Hello, Netters.
>: Is there any one heard of the program manuplate plasmid sequence?
>: I want to make a program simulate plasmid manuplation.
>: display restriction fragments and plasmid map.
>: make result plasmid sequence. and so on..
>: like Restriction with EcoRI and elute small fragmet.
>: and then Blunting with Klenow fragment of DNA Pol.
>: and ligating with SmaI digested vector.
>: then what result is expected?
>: Is this stratagy valid?
>: any advice is welcome.
>: thanx in advance.
>:>: Son Hosun
>: Mol. Bio.
>: Seoul National Univ.
>:hosun at alliant.snu.ac.kr
>Yes, that is a very useful program. It has already been written for the
>Macintosh computers (Gene Construction Kit by Textco Incorporated) but
>I have not yet seen it for IBM or UNIX.
>Most plasmid programs only draw maps, they do not also carry along the
>sequence. The GCK is the only one to my knowledge that does both.
> (SIG)
If you're working in the Windows environment, There is a program that does all
this. It is called Gene Runner, and is sold by Hastings Software, Inc. POBox
567 Hastings on Hudson, NY 10706. Tel (800)834-8574, Fax (914)376-4433.
It is very powerful, and a demo is available. Only pitfall is the price: $399
is their academia discounted price (!)
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Ron Caspi Scripps Institution of Oceanography UC San Diego
La Jolla CA 92093-0202 Phone (619)534-0638 Fax (619)534-7313
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