Is there any one heard of the program manuplate plasmid sequence?
I want to make a program simulate plasmid manuplation.
display restriction fragments and plasmid map.
make result plasmid sequence. and so on..
like Restriction with EcoRI and elute small fragmet.
and then Blunting with Klenow fragment of DNA Pol.
and ligating with SmaI digested vector.
then what result is expected?
Is this stratagy valid?
any advice is welcome.
thanx in advance.
Seoul National Univ.
hosun at alliant.snu.ac.kr