densitometer information

gilles.bronchti at ia.unil.ch gilles.bronchti at ia.unil.ch
Fri Sep 17 01:27:56 EST 1993

I have only a little experience in analysing sequencing gels, 
I did that only once for a friend, but I am using quite extensively 
densitometry for analysing 2DG autoradiograms of brain sections. For that 
I use the NIH-Image software, on a McIntosh Quadra 950 computer, coupled 
to a video camera Sony XC-77 with a frame grabber card DATA TRANSLATION 
I must say that I am very happy with this set up. The camera is fixed 
on a macroscope (can be fixed also on a microscope or used alone with its 
own objectives). The software Image is a great thing. Extremely powerful and versatil. 
It has a lot of macros procedures for gel analysis, autoradiography, morphometry.
Very easy to use. It is a public domain software updated almost every
month with new improvements and features. As I said we are using it for 
densitometric analysis but also for morphometric studies (diameter and area of axons,
size of injection sites, counting of cells and so ....).
Is it the best, I don't know, but it is free, updated, friendly, and versatile.
It has also a "living-paste" procedure that allows you to align serial sections or plots,
a "stack" procedure for 3D-reconstructions, and allows use of pseudocolor with
a great number of look-up tables.
If you need more information, contact me.
Gilles.bronchti at ia.unil.ch

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