> In article <2ctjnm$luk at triton.unm.edu>, <dkim at unm.edu> wrote:
> >Is there any shareware/PD software for doing "densitometry" on a
scanned
> >image of a gel or autoradiogram (e.g. a TIFF file)? Is this very
complicated
> >or kind of trivial to do?
>> BandLeader, a MS-Windows program (from ftp.bio.indiana.edu, or its
> Gopher) will do that. It does its bit from a BMP file. The only
> problem I have with BandLeader is that ... there is no way of
printing the trace
> from within the program. Otherwise, I like the program, it's very
> nice ---> impressions from the last 2-3 hours of use. It's
_really_
> trivial to use:
>> i. Scan the gel or whatever into a bmp file,
> ii. Pull bmp file into Bandleader,
> iii. Do a "profile"
> iv. (optional) edit the trace to remove noise.
>> That's it.
...and you can manipulate, "normalise", etc. said trace, and you can
do comparative densitometry (=integration) on two or more bands
simultaneously, using the "grid" option - actually works in telling
whether a band in the sequencing gel that comes with BL is a doublet
or singlet.
And you CAN print the densitometry output: just do an Alt + PrtScrn
when the window is active, to send only the profile window to
the clipboard, paste into your favourite shareware graphics package
(mine is PaintShop Pro), and print!
A fine doodad, kudos to Ma'ayan Aharoni!
____________________________________________________________________
| Ed Rybicki, PhD | |
| (ed at micro.uct.ac.za) | "Lord, won't you buy me |
| Dept Microbiology | A Mer-ce-des Benz |
| University of Cape Town | My friends all have Porsches |
| | I must make amends..." | |
| Private Bag, Rondebosch | |
| 7700, South Africa | - Janis Joplin |
| fax: 27-21-650 4023 | (Pearl, 1971) |
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