In article <1993Feb5.122833.1 at hkucc.hku.hk> vldnaseq at hkucc.hku.hk writes:
>Dear netters,
>>I am optimizing the hybridization and washing conditions for single base
>mismatch oligonucleotide hybridization experiments. This is used as a means
>to detect mutations. Is there any software that can predict accurately the
>Tm of a complete match / single mismatch under different ionic strengths ?
>>Wai Ming
A quick Gopher seacrh tells me that there is a program for the PC
called Melt (by John Nash) which may be what you're looking for.
You can obtain melt.uue by gopher or by anonymous ftp at
felix.embl-heidelberg.de.
Best of luck,
Dan Jacobson
danj at welchgate.welch.jhu.edu
