Can anyone help me to calculate free Ca2++ in EGTA/CA buffers? Basically
I want to determine the effect of Ca on enzyme activity, and relatedly
translocation of an enzyme to membranes-which is Ca -dependent. I need
to include EGTA to completely inhibit the enzyme, and then add back Ca.
This must have been done thousands of times but I am completely
bamboozeld by the books I've looked at. What I need is a simple
explanation and perhaps a program, spreadsheet or Hypercard stack
for the calculations (so Mac, or PC Excel). Thanks.
FErgus Doherty, Biochemistry, Nottingham University, UK.