Calcium buffer program available for ftp

kleinschmidt at mcclb0.med.nyu.edu kleinschmidt at mcclb0.med.nyu.edu
Wed Sep 16 22:26:45 EST 1992

                         CABUFFER program

The program CABUFFER is available by anonymous ftp from 
mcclb0.med.nyu.edu [].  This program allows you to 
calculate the concentrations of all ionic species present in a mixture 
of up to four divalent cations (e.g. Mg, Ca, Sr, Ba) and four ligands 
("buffers") for these ions.  Examples of such buffers are EDTA, EGTA, 
NTA, HEDTA, citrate, Ca-binding proteins, etc.  Corrections for 
temperature, ionic stength and pH are provided.  

The program is written in structured Basic and has been compiled with 
Borland's Turbo Basic v. 1.0.  It can also be compiled with Microsoft's 
Quickbasic v. 4.0 or run from within the QBASIC program provided with 
MS-DOS 5.0 or higher, after making a few changes (see the readme file).  
The compiled program runs on PC's under PC-DOS/MS-DOS 2.0 or higher.

It runs very fast on PC's outfitted with a math coprocessor.  A somewhat 
less capable program, CABUF, that runs reasonably fast even on machines 
without math coprocessor, is also available.  Both executable files and 
Basic source code is included.  All the files take up about 280 Kb of 
space, including a readme file that tells you how to use the program.  
The CABUFFER.EXE file is about 80 Kb long.  

Originally, I meant to write a paper on the use of Ca-buffers in 
physiology and on the traps one can fall into using them.  The 
manuscript was never completed but drafts of various sections are 
included with CABUFFER.  They may make instructive reading for those who 
care to understand what they are doing when they make up and use 
Ca-buffers.  RESULTS.TXT, in particular, describes the basic aspects of 
the CABUFFER program, including the corrections utilized.  I apologize 
for the fact that equations are missing from the electronic version of 
the manuscript; I couldn't get them into ASCII format.
Unfortunately, I have little time to support this program.  If you use 
it, you do so at your own risk.  But I believe that its use is 
self-explanatory and easy, and I also think that it is largely bugfree.

The program is supplied in two files:

CABUFZIP.EXE: This is a binary, self-extracting, compressed archive file 
that contains all program, data and text files.

CABUFZIP.RME: A short readme file that gives installation instructions 
for CABUFFER.  This file is reproduced below.

FTP instructions

At the command prompt of your machine, type: 


This will connect you to my host machine (mcclb0.med.nyu.edu).  Follow 
the prompts as shown below.

Name: anonymous
Password: your_user_name
ftp> binary
ftp> get cabufzip.exe
ftp> ascii
ftp> get cabufzip.rme
ftp> quit


CABUFZIP.RME (9-12-92)

Installation instructions for CABUFFER on MS-DOS machines

The compiled CABUFFER program, Basic source code files, data files, and 
explanatory text files all reside in one self-extracting, compressed 
archive file named cabufzip.exe.

To install all of these files on your PC-DOS/MS-DOS machine, at the ftp 
prompt type BINARY <enter> to set the transfer mode to binary, then 
transfer the file with the command GET CABUFZIP.EXE <enter>.  Once this 
file is on your PC, all you have to do is to execute it with the command 
CABUFZIP <enter>, preferably in its own subdirectory.  This will extract 
and unpack the compressed component files.  The cabufzip.exe file is 125 
kB long, the extracted files add up to about 280 kB.  CABUFFER itself is 
80 Kb long.  The user notes for CABUFFER are in the readme file.

Jochen Kleinschmidt, Ph.D.
Dept. of Ophthalmology
NYU Medical Center

Phone:    212-263-5530
Internet: kleinschmidt at mcclb0.med.nyu.edu
Bitnet:   kleinschmidt at nyumed.bitnet

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