NCSA GelReader v2.0.3 has been released! It is available via anonymous
FTP (ftp.ncsa.uiuc.edu or 141.142.20.50) in the Mac/GelReader directory;
via the archive server (archive-server at ncsa.uiuc.edu); or via the
Technical Resource Catalog. Catalogs may be obtained by contacting:
NCSA
University of Illinois at Urbana-Champaign
Attention: Documentation Orders
152 Computing Applications Building
605 E. Springfield Ave.
Champaign, IL 61820
Please send any questions or comments to softdev at ncsa.uiuc.edu
and any bug reports to bugs at ncsa.uiuc.edu.
The following is a copy of the README file:
Congratulations on your acquisition of NCSA GelReader 2.0.3 for the
Macintosh! Note that there are two different versions of this
program: one for machines that have not floating point processor,
and one for machines that do.
NCSA GelReader is a product designed to automate to some degree
the measurement of DNA length using digitized electrophoretic gels.
First, a digitized gel image is read into GelReader, and displayed as an
image. GelReader has the capability to find the lanes and the bands
in the gel. Lanes and bands can be interactively added and deleted if
necessary. Then, known molecular weights (standards) can be used
to approximate the molecular weight of the remaining molecules. The
result is a text report listing the approximated molecular weights of
the molecules.
To get started, you will need to open the UnStuffit application to
decompress the GelReader application, documentation, and sample
files.
NEW FEATURES IN VERSION 2.0
This version has been completely rewriten, and now provides a much
more intuitive user interface. Creating, deleting and editing
standards is now much easier, and assigning standards to lanes in a
gel has been greatly improved.
Profiles now appear in the same window with the gel. You may
superimpose several profiles in the same view. You can also save a
gel file, its' lanes and bands and its notebook into an HDF file. When
you open the HDF file again, you will see all the lanes and bands that
were there when you saved it, and the notebook will still be intact.
Multiplexing is also supported now. You can run standards and
unknowns in the same lane, probe for the knowns and generate the
image, then probe for the unknowns and generate a separate image.
The results of the analysis of the image with the knowns can then be
superimposed onto the image with the unknowns.
And much, much more.
FIXES
in 2.0.2:
1) There was a bug that would cause the program to crash whenever
you manually added more than one lane. Fixed!
2) It no longer reports standards.
3) It would sometimes report than the program was out of memory
when using the copy lanes command. Fixed.
4) It no longer crashes when you try to generate a report and you
have unknowns above internal controls (knows imbedded in the
lane.
5) Computations for unknowns outside of the range of the knowns
are still less accurate, but tend to be much more reasonable now,
and the fact that these computation are less accurate are flagged
in the report.
in 2.0.3:
1) it no longer refreshes the entire screen when deleting or adding
bands.
2) I can no longer reproduce the file save problems, although I don't
know why.
WHAT THIS DISK CONTAINS
GelReader2.0.3LCsi.sit compressed GelReader application(No FPU)
or
GelReader2.0.3mac.sit compressed GelReader application
Samples.sit compressed sample files
README this file
UnStuffit 1.51 decompression utility
Thomas Redman
Project Leader
National Center for Supercomputing Appls.
152 Computing Applications Building
Champaign, IL 61820
