Program Name: sg2iq.exe
Program Documentation: sg2iq.doc
Operating Environment: PC running DOS
Purpose of Program: Utility program for converting images of gels created by
software for a Hewlett Packard desktop scanner (tiff files) to the format
expected by the ImageQuant gel analysis software from Molecular Dynamics.
Program Usage: sg2iq inputfile outputfile
where "inputfile" is produced by the Hewlett Packard scanner (extension .tif)
and "outputfile" is a file that can be used by ImageQuant (extension .gel).
Program Author: David Rigney, Ph.D., Harvard/M.I.T. Division of Health Sciences
and Technology and the Harvard Division on Aging
To obtain the program: anonymous ftp to amber.mgh.harvard.edu (132.183.190.26)
ftp amber.mgh.harvard.edu
username: anonymous
password: <your email address>
cd pc
get sg2iq.doc
binary
get sg2iq.exe
quit
Discussion: The ImageQuant software from Molecular Dynamics is ordinarily
obtained when you purchase one of their phosphorimagers. It runs under
Microsoft Windows and allows you to quantify objects found in gel images.
The software can also be purchased by itself. You may also need to image
film autoradiographic images, wet silver stained gels, photographic negatives
of ethidium bromide stained gels, etc., for example to normalize for loading
variability in different gel lanes. An inexpensive way to image these objects
is to use a desktop publishing scanner from Hewlett Packard. Use the Scanning
Gallery software that is provided with the scanner to produce a .tif file,
then use sg2iq (Scanning Gallery To Image Quant) to obtain a file that the
Image Quant software can use.
Posted on the bio-software bulletin board of bionet, October 1992.
