What Genomes have been Sequenced?

Francis Ouellette francis at monod.Biol.McGill.CA
Mon Oct 12 10:31:46 EST 1992

Jean-Loup Risler (BIONET at FRCGM51.EARN) e'crit: 

>1) The "landmark paper" on Yeast Chromosome III appeared in NATURE, vol. 357
>   pp. 38-46 and not in Science. I'm not quite sure whether the cited
>   number of authors (>100) was ironical or not. If so, why (I'm not a co-
>   author)?

147 authors from 35 labs in Europe.  If I was one of those labs, I sure
would want my name on it.  This papers, and others in the near future to
come from Europe shows one way to do a mini-mega sequencing project.
Many (in the human and C. elegans) project have made fun of the Yeast
project but the Nature paper has silenced many, and it works!  We can
sequence a small genome (14,000,000 bp +/-) by "conventional" cloning and
sequencing methods.  They will not be able to do this for the human genome
project ... they will need new technology.

>2) Keith Robison has forgotten *Bacillus subtilis* in his review of partly

Keith also mentions 3 yeast chromosome on their way to completion ...  it
is more like 4 ...   I, II, III (finished) and XI.

>3) About a former posting: in yeast chromosome III, there are 182
>   open reading frames longer than 300 bp (thus coding for proteins longer

and there are probably more, and some of the already identified ones may
be bogus.  Many carreers are spent on a single gene ... so more than 180
for CHIII and more than a thousand (2000?) for the yeast genome will keep
many many people very busy.  The task of genome sequencing is very
altruistic (well, I think it is ;-).  We are producing data which will be
used (and corrected!) by thousands of scientist worldwide, and not just
yeast biologists.

>That more than 50% of the ORFs in ChrIII do not resemble anything
>previously known is one great information afforded by its sequencing.
>Remember also that yeast is a small eukaryotic organism, whose genome
>contains introns and exons, and that reverse genetic experiments are easy
>with yeast. The choice of this organism for a collaborative project was not
>done by chance...

I think you meant very few introns here (?) ...   and yes, not much
"chance" in choosing yeast here ... if I can quote somebody from my yeast
mailling list (yes, yes, it is comming folks ;-) ...

 "Yeast is Best"

regards to all


(PS maybe this should move from bionet.software to bionet.general?)
(PPS salut Jean-Loup!)

| B.F. Francis Ouellette  
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis at monod.biol.mcgill.ca

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