Hello,
Does anybody know of software to calculate the pI of peptides? I have a
colleague who runs the departmental peptide synthesis/sequencing unit. Some of
his longer peptides (>30residues) are difficult to clean up by RPHPLC, so
ion-xchange HPLC might be better. If he knew the expected pI of the full
length paptide and the truncated contaminants he could design a separation. I
suppose you could do this with a speardsheet but is their a PD program, Mac or
PC?
Presumably there are difficulties with predicting pI if there is any secondary
structure in the longer paptides. Thanks.
Fergus Doherty,
Dept. Biochemistry,
Nottingham University, UK.
