We have digitized images of a video camera, which are black and
white in approx. 500 * 500 resolution. The image is an electron micrograph
prepared by "rotary shadowing". This gives a very coarse grain of
spots of the size of up to 4x4 pixels, and round shape. The object we are
interested in is a lengthy rod, of about 100x6 pixels, which is bended and
looks like a worm. There are multiple of these worms on each image, but they
don't interfere. For measuring, we would like to have the coordinates of the
midpoint of the bended rods.
Question: Given a TIFF or other popular image format, is there a software
which will filter out the grainy background, and determine the coordinates
in the form of approx. 100 coordinate pairs per worm?
Thanks
Reinhard
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Dr. Reinhard Doelz * EAN doelz at urz.unibas.ch
Biocomputing * DECNET 20579::48130::doelz
Biozentrum der Universitaet * X25 psi%022846211142::embnet
Klingelbergstrasse 70 * FAX x41 61 261- 6760
CH 4056 Basel * TEL x41 61 267- 2076 or 2247
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