G'day folk,
I saw this today...
*Date: Mon, 13 May 91 18:34 CDT
*From: Bruce Roe <BROE at aardvark.ucs.uoknor.edu>
*Subject: re: pUC vectors
*To: bio-soft at genbank.bio.net
Shouldn't this be "methods @genbank.bio.net"? Not a complaint from me,
just that it may get a more aappropriate audience there.
*X-Vms-To: IN%"bio-soft at genbank.bio.net"
*You wrote:
*>> Hi there,
*>> we are looking for a simple pUC vector (preferably pUC 18
*>> or 19) with a T7 promoter and a T3 promoter cloned in the
*>> polylinker.
*>> We already have pUC118, but this vector contains, as you know,
*>> an origin for single stranded replication, and this hampers
*>> our cloning scheme.
Shortened for brevity...
and
*>But what we really want is a vector that looks like this: pUC sequences-
*>T7 promoter-polylinker-T3 promoter-pUC sequences. Simple ? Yes !
*>M13 ori for ss replication ? No !
and
* Bethesda Research Labs. has to vectors called:
*pT7/T3a-18 and pT7/T3a-19. or at least they do according to their
*catalogue. They both are pUC bases and have either the pUC18 or 19
*multiple cloning site flanked by the T7 and 73 promoters.
I use the pT7/T3-alpha vectors, and I hate to report that they have
M13 (or f1) ss ori sites.
Sorry to be a bubble burster, but I think that Clemens may have to
start constructing ;-(
cheers,
John,
--------------------------------------------------------------
John H.E. Nash <Bitnet: NUM208JN at NRCCAD.NRC.CA >
Institute for Biological Sciences,
National Research Council of Canada,
Ottawa, Canada K1A 0R6.
Phone: (613) 990-0990 Fax: (613) 952-9092.
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P.S. It's a BOY!
