I could not help giving my 2 cents worth regarding the posting on pUC vecter.
It seems to me that people of the electronic age are even more narrowly-minded
than their "ancestors". Maybe they are lazier too.
Answer to the question is the pBS vector from Strategene. Even if one doesn't
have the money to buy it, one could still do some simple restriction digestion
and ligation to satisfy the experimental need [since the pUC based vector is
available to the questioneer, with an unwanted feature, right?].
Problems tend to be unique for individual project, customerization usually
would be the only way out. To be a little deligent and broad-minded will
always help. Another thing is whoever answering the posting is actually doing
the questioneer a favor. I hope that everyone could keep this in mind.
Tao Tao "My name has nothing to do with the
Graduate Student Taoism, even though I am a believer of it."
Department of Microbiology
Medical College of Ohio
Toledo, Ohio 43699-0008