You wrote:
>> Hi there,
>> we are looking for a simple pUC vector (preferably pUC 18
>> or 19) with a T7 promoter and a T3 promoter cloned in the
>> polylinker.
>> We already have pUC118, but this vector contains, as you know,
>> an origin for single stranded replication, and this hampers
>> our cloning scheme.
>> If you have this kind of vector, we would be very pleased to hear
>> from you.
>> Christian and Clemens
>this message is apparently very confusing, since we were recieved many
>answers (for which we are grateful) that we should use bluescript (or
>could it be that only few people know that this vector contains an ori
>for single stranded replication ?).
>But what we really want is a vector that looks like this: pUC sequences-
>T7 promoter-polylinker-T3 promoter-pUC sequences. Simple ? Yes !
>M13 ori for ss replication ? No !
>Before I start constructing myself, I would like to hear from anybody out
>there, whether he has this rather simple construct, or one like it.
>Clemens
Bethesda Research Labs. has to vectors called:
pT7/T3a-18 and pT7/T3a-19. or at least they do according to their
catalogue. They both are pUC bases and have either the pUC18 or 19
multiple cloning site flanked by the T7 and 73 promoters.
Cheers,
****************************** Bruce A. Roe ******************************
* Professor of Chemistry and Biochemistry Dept. of Chem. and Biochem. *
* INTERNET: BROE at aardvark.ucs.uoknor.edu University of Oklahoma *
* BITNET: BROE at uokucsvx 620 Parrington Oval, Rm 208 *
* AT&TNET: 405-325-4912 or 405-325-7610 Norman, Oklahoma 73019 *
* FAXnet: 405-325-6111 *
****************************************************************************
* ICBMnet: 35 deg 14 min North, 97 deg 27 min West *
****************************************************************************
