Hello again...
I recently posted a question on calculating protein extinction coefficients.
I received a couple good replies, but I actually got the best information
over the phone from Irv Edelman at GCG. He recommended a paper by Gill
and von Hippel (Anal. Biochem. 182:319-26. 1989) which goes into the both
the calculation on extinction coefficients at 280nm, as well as their
significance WRT the real experimentally determined values.
I then used their calculation (quite simple in fact) to write a little
snippet of code that I put into the GCG program peptidesort. Now I have
a little program that calculates extinction coefficients as well as all
the other nice things peptidesort did before.
If any one is interested in my little snippet of code, drop me a line and
i can send it. According to Irv, Peptidesort will do these calculations
in release 7, so don't feel that you need to modify your version right away
;)
Thanks again for the info...
Keith
uunet!av8tr!elliston
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Keith O. Elliston | .signature really under construction....
uunet!av8tr!elliston |
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