Am posting for a friend. He needs a program that he can run on his pc to
estimate band sizes on Southern blots. His description was sketchy, bear
with me.
Currently, he uses a prg written in quick basic, in conjunction with a
digitizing pad. His program works like this. Put the southern blot on
the pad (visualization done by biotin labelling), and then he touches the
pointer to the origin in lane #1, then on each band in lane #1, which is
MW markers. He enters the band sizes, and the prg figures a std curve,
basically a plot of fragment size vs. migration distance. From this, he
can estimate band sizes in the following lanes.
His problem is that he loses accuracy with fragments larger than 7 kb.
Anyone familiar with any programs that he can get? I know that there are
some nice *expensive* machines that do this, so if any of you have any
info or experience using them, your input is welcome as well.
Hopefully,
dan
__________________________________________________________________________
**** The shallow man has *** | Do not be overly righteous, nor overly
**** has opinions; *** | wise: why should you destroy yourself ?
**** the deep, *** | - King Solomon, wisest man of his
**** convictions. *** | day, Ecclesiastes 7:16.
