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Gary Williams x3294 gwilliam at mrc-crc.ac.uk
Wed Jan 31 10:19:03 EST 1990

Does anyone know of a program to aid the selection of regions of a
nucleic acid sequence to act as good primers for PCR?

Ideally, the regions required should have a high Td so that they do not
anneal to themselves.  They should form stable duplexes with and be
specific to, the target sequence.  They should not base-pair to other
regions on the target sequence.

Gary Williams

Computing Services Section,		 Janet:       G.Williams at UK.AC.CRC
MRC-Clinical Research Centre,		 Elsewhere:   G.Williams at CRC.AC.UK
Watford Rd, HARROW, Middx, HA1 3UJ, U.K. EARN/Bitnet: G.Williams%CRC at UKACRL
Tel 01-869 3294    Fax 01-423 1275       Usenet: ...!mcvax!ukc!mrccrc!G.Williams

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