On 22 Feb '90, Dan Davison <davison at UHNIX2.UH.EDU> sent the following:
DD>Subject: need PD probe (Tm), oligo pgm
DD>A student in the department needs a program which will calculate
DD>oligo Tms for making probes for sequencing a region which is (1)
DD>highly repetitive and (2) unreachable by Sequinase (tm?).
Do you still need such a prog? I have just written one for the IBM-PC
family of computers which will calculate melt_temps for DNA sequences of
14 to 80 bases using the algorithm on page 11-46 of Sambrook et al.,
It will accept degeneracies, treat them as mismatches, reducing the
melt_temp by 1 degree for every 1% mismatch. Sorry, but it's for MS-DOS
computers only.
If you need it, let me know....
cheers,
John,
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John H.E. Nash <bitnet/earn: NUM208JN at NRCCAD.NRC.CA >
Division of Biological Sciences,
National Research Council of Canada,
Ottawa, Canada.
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Disclaimer: All opinions expressed here are MINE, and
do NOT necessarily reflect the opinions held
by the National Research Council of Canada!!
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